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Development of a microplate method for the determination of hepatic UDP-glucuronosyltransferase activity in rainbow trout (Oncorhynchus mykiss) and Nile tilapia (Oreochromis niloticus)

Authors: Jamie M Hooft, Yanping Lou, E James Squires, John P Cant, Dominique P Bureau

Publication Date: 2021/10/1

Hepatic glucuronidation represents an important phase II biotransformation reaction in both mammals and fish. The kinetic characteristics of uridine 5′-diphosphate (UDP) glucuronosyltransferases (UDPGTs) in rainbow trout liver microsomes were examined using p-nitrophenol (p-NP) as an aglycone and UDP-glucuronic acid (UDPGA) as a glucuronyl donor according to an existing protocol. 

Read it here: https://www.sciencedirect.com/science/article/abs/pii/S1532045621001411